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Bio-Rad Crime Scene Investigator PCR Basics™ Kit

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 Product Description

Part Number 36-5207

Bio-Rad Crime Scene Investigator PCR Basics™ Kit

Renowned as a premier name in biotechnology education, Bio-Rad Laboratories offers research-level lab activities that are easily accessible to high school and college students. Each exemplary experiment is designed to bring trusted, reliable, repeatable results to your lab. Now, with the expertise of Bio-Rad, you can provide your students with authentic activities and apparatus that fully prepare them for future biotechnology study, applications, research, and careers.

Each Bio-Rad Kit features:

  • Award-winning, real-world science
  • Proven materials and methods
  • Curriculum manual with teacher and student guides
  • Supplies and techniques used in today's most advanced biotech labs
  • Materials to support 32 students working in groups of various sizes

Using real-world forensic lab techniques, students learn which human DNA sequences are used for crime scene, missing person, mass disaster, and paternity investigations. Using polymerase chain reaction (PCR) and DNA electrophoresis, students determine the genotypes of five DNA samples and perform a simple statistical analysis on their own results to solve a forensic mystery. DNA profiling determines the exact genotype of a DNA sample and distinguishes one human being from another by identifying a DNA “bar code” that is unique to each individual. Within the variant areas of the genome exist unique polymorphic sequences called short tandem repeats (STRs) of uncertain biological function. It is these DNA sequences that are used in forensic applications. The DNA samples contained in this introductory PCR kit are plasmids that have been engineered to mimic the natural variations in human DNA that exist between one human being and another at a single STR locus. Complete the lab in two 45 minute lab sessions. Includes materials for eight setups.

Kit Contents:

  • Crime scene DNA sample, 250 μl
  • Suspect A DNA sample, 250 μl
  • Suspect B DNA sample, 250 μl
  • Suspect C DNA sample, 250 μl
  • Suspect D DNA sample, 250 μl
  • PCR master mix (Taq DNA polymerase, dNTPs, buffer), 1.2 ml
  • Primers (blue), 25 μl
  • Allele ladder, 200 μl
  • Orange G loading dye, 1 ml
  • PCR tubes, 0.2 ml
  • Capless PCR tube adaptors, 1.5 ml
  • Colored microcentrifuge tubes, 2.0 ml
  • Foam floats
  • Curriculum including teacher’s guide, student manual, and graphic quick guide

Required Accessories, Not Included in Kit:

  • Horizontal gel electrophoresis chambers
  • Adjustable micropipets (2–20 μl, 20–200 μ)
  • Pipet tips, aerosol barrier (2–20 μl, 20–200 μl)
  • Microcentrifuges
  • Thermal cycler
  • Power supplies

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